Sensitive and quantitative universal Pyrosequencing methylation analysis of CpG sites.
نویسندگان
چکیده
منابع مشابه
Quantitative detection of DNA methylation states in minute amounts of DNA from body fluids.
Quantitative and reliable estimation of DNA methylation levels for multiple genomic regions pose a major challenge where starting DNA is available in very low quantity. Here we review major advances in the development of techniques for quantitative detection of DNA methylation in minute amount of DNA and describe a detailed protocol for quantitative Methylation Analysis of Minute DNA amounts af...
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BACKGROUND New high-throughput sequencing technologies promise a very sensitive and high-resolution analysis of DNA methylation patterns in quantitative terms. However, a detailed and comprehensive comparison with existing validated DNA methylation analysis methods is not yet available. Therefore, a systematic cross-validation of 454 sequencing and conventional pyrosequencing, both of which off...
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عنوان ژورنال:
- BioTechniques
دوره 35 1 شماره
صفحات -
تاریخ انتشار 2003